santa cruz sc (Santa Cruz Biotechnology)
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Santa Cruz Sc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 585 article reviews
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Incubation:Article Title: Lack of ANKMY2 suppresses kidney cystogenesis in embryonic- and adult-onset polycystic kidney disease Article Snippet: Sections were then blocked in blocking buffer (1% normal donkey serum [Jackson immunoResearch, West Grove, PA], in PBS) for 1 hour at room temperature. .. Sections were incubated with primary antibodies against the following antigens; overnight at room temperature or 4C: Acetylated tubulin (T6793; Sigma mouse IgG2b, 1:500), Article Title: Lack of ANKMY2 suppresses kidney cystogenesis in embryonic- and adult-onset polycystic kidney disease. Article Snippet: Sections were then blocked in blocking buffer (1% normal donkey serum [Jackson immunoResearch, West Grove, PA], in PBS) for 1 hour at room temperature. .. Sections were incubated with primary antibodies against the following antigens; overnight at room temperature or 4C: Acetylated tubulin (T6793; Sigma mouse IgG2b, 1:500), Article Title: Diurnal function and expression of aquaporins in the mouse kidney. Article Snippet: The membranes were blocked for 1 148 h at room temperature in a mixture of equal parts of protein blocking buffer (Li-Cor, Lincoln, NE) 149 and tris-buffered saline (TBS; 20 mM Tris, 150 mM NaCl, pH 7.6) while being gently shaken. .. 150 Next, the membranes were incubated with primary antibodies against AQP1 (Proteintech, 151 20333-1-AP, rabbit polyclonal, 1:5000) (32), Article Title: Diurnal function and expression of aquaporins in the mouse kidney Article Snippet: The membranes were blocked for 1 h at room temperature in a mixture of equal parts of protein blocking buffer (Li-Cor, Lincoln, NE) and tris-buffered saline (TBS; 20 mM Tris, 150 mM NaCl, pH 7.6) while being gently shaken. .. Next, the membranes were incubated with primary antibodies against AQP1 (Proteintech, 20333–1-AP, rabbit polyclonal, 1:5000) ( 32 ), Immunofluorescence:Article Title: The critical role of the proto-oncogene c- Kit in TSC renal cystogenesis Article Snippet: The following antibodies were utilized for immunofluorescence (IF) or immunohistochemistry (IHC) and Western blots (WB): (1) p-ribosomal S6--IHC (1:100) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (2) c-KIT antibody--IF (1:100) and WB (1:1000) (Cell Signaling Technology; Danvers, MA); (3) pERK1/2 IHC (1:300) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (4) pRSK1 IHC (1:100) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (5) ser1798 p-TSC2 IF (1:75) and WB (1:800) (ThermoFisher Scientific, Eugene, OR); and (6) pAKT IHC (1:50) and WB (1:1000) (Cell Signaling Technology, Danvers, MA). .. The following antibodies were used for immunofluorescence studies: (1) Monoclonal H + -ATPase (1:50; Santa Cruz Biotechnology); (2) H + -ATPase (1:75; our lab); (3) PCNA (1:50; Santa Cruz Biotechnology); and (4) Article Title: The critical role of the proto-oncogene c-Kit in TSC renal cystogenesis. Article Snippet: The following antibodies were utilized for immunofluorescence (IF) or immunohistochemistry (IHC) and Western blots (WB): (1) p-ribosomal S6--IHC (1:100) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (2) c-KIT antibody--IF (1:100) and WB 14 EMBO Molecular Medicine © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply (1:1000) (Cell Signaling Technology; Danvers, MA); (3) pERK1/2 IHC (1:300) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (4) pRSK1 IHC (1:100) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (5) ser1798p-TSC2 IF (1:75) and WB (1:800) (ThermoFisher Scientific, Eugene, OR); and (6) pAKT IHC (1:50) and WB (1:1000) (Cell Signaling Technology, Danvers, MA). .. The following antibodies were used for immunofluorescence studies: (1) Monoclonal H+-ATPase (1:50; Santa Cruz Biotechnology); (2) H+-ATPase (1:75; our lab); (3) PCNA (1:50; Santa Cruz Biotechnology); and (4) other:Article Title: A Novel Human Distal Tubuloid-on-a-Chip Model for Investigating Sodium and Water Transport Mechanisms Article Snippet: , |

