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santa cruz sc  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology santa cruz sc
    Santa Cruz Sc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aqp2/AQP2+Antibody/pm41930611-90-88-88
    Average 96 stars, based on 585 article reviews
    santa cruz sc - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Lack of ANKMY2 suppresses kidney cystogenesis in embryonic- and adult-onset polycystic kidney disease
    Article Snippet: Sections were then blocked in blocking buffer (1% normal donkey serum [Jackson immunoResearch, West Grove, PA], in PBS) for 1 hour at room temperature. .. Sections were incubated with primary antibodies against the following antigens; overnight at room temperature or 4C: Acetylated tubulin (T6793; Sigma mouse IgG2b, 1:500), AQP2 (SC515770, Santa Cruz Biotechnology, mouse IgG1, 1:500), Ki67 (ab16667, Abcam, 1:500), pCREB (9198S; Cell Signaling, 1:500). .. After three PBS washes, the sections were incubated in secondary antibodies (Alexa Fluor 488-, 555-, 594-, 647- conjugated secondary antibodies, or anti-mouse IgG isotype-specific secondary antibodies; 1:500; Life Technologies, Carlsbad, CA or Jackson ImmunoResearch) or cell surface markers, Fluorescein labeled Lotus tetragolonobus lectin (LTL; 1:200, FL 1321–2 Vector laboratories) for 1 hour at room temperature.

    Article Title: Lack of ANKMY2 suppresses kidney cystogenesis in embryonic- and adult-onset polycystic kidney disease.
    Article Snippet: Sections were then blocked in blocking buffer (1% normal donkey serum [Jackson immunoResearch, West Grove, PA], in PBS) for 1 hour at room temperature. .. Sections were incubated with primary antibodies against the following antigens; overnight at room temperature or 4C: Acetylated tubulin (T6793; Sigma mouse IgG2b, 1:500), AQP2 (SC515770, Santa Cruz Biotechnology, mouse IgG1, 1:500), Ki67 (ab16667, Abcam, 1:500), pCREB (9198S; Cell Signaling, 1:500). .. After three PBS washes, the sections were incubated in secondary antibodies (Alexa Fluor 488-, 555-, 594-, 647- conjugated secondary antibodies, or anti-mouse IgG isotype-specific secondary antibodies; 1:500; Life Technologies, Carlsbad, CA or Jackson ImmunoResearch) or cell surface markers, Fluorescein labeled Lotus tetragolonobus lectin (LTL; 1:200, FL 1321–2 Vector laboratories) for 1 hour at room temperature.

    Article Title: Diurnal function and expression of aquaporins in the mouse kidney.
    Article Snippet: The membranes were blocked for 1 148 h at room temperature in a mixture of equal parts of protein blocking buffer (Li-Cor, Lincoln, NE) 149 and tris-buffered saline (TBS; 20 mM Tris, 150 mM NaCl, pH 7.6) while being gently shaken. .. 150 Next, the membranes were incubated with primary antibodies against AQP1 (Proteintech, 151 20333-1-AP, rabbit polyclonal, 1:5000) (32), AQP2 (Santa Cruz, sc-9882, goat polyclonal, 152 journals.physiology.org/journal/ajprenal (036.088.103.197) on October 9, 2025 .. 9 1:1000) (33), phosphorylated S256 AQP2 (Abcam, ab111346, rabbit polyclonal, 1:1000) (34), 153 phosphorylated S261 AQP2 (PhosphoSolutions, p112-261, rabbit polyclonal, 1:1000) (35), 154 AQP3 (rabbit polyclonal, final bleed, 1:1000) (33), AQP4 (Alomone Labs, AQP-004, rabbit 155 polyclonal, 1:1000) (36), NKCC2 (StressMarq, SPC-401D-FITC, rabbit polyclonal, 1:300) (37).

    Article Title: Diurnal function and expression of aquaporins in the mouse kidney
    Article Snippet: The membranes were blocked for 1 h at room temperature in a mixture of equal parts of protein blocking buffer (Li-Cor, Lincoln, NE) and tris-buffered saline (TBS; 20 mM Tris, 150 mM NaCl, pH 7.6) while being gently shaken. .. Next, the membranes were incubated with primary antibodies against AQP1 (Proteintech, 20333–1-AP, rabbit polyclonal, 1:5000) ( 32 ), AQP2 (Santa Cruz, sc-9882, goat polyclonal, 1:1000) ( 33 ), phosphorylated S256 AQP2 (Abcam, ab111346, rabbit polyclonal, 1:1000) ( 34 ), phosphorylated S261 AQP2 (PhosphoSolutions, p112–261, rabbit polyclonal, 1:1000) ( 35 ), AQP3 (rabbit polyclonal, final bleed, 1:1000) ( 33 ), AQP4 (Alomone Labs, AQP-004, rabbit polyclonal, 1:1000) ( 36 ), NKCC2 (StressMarq, SPC-401D-FITC, rabbit polyclonal, 1:300) ( 37 ). .. The primary antibodies were diluted in protein blocking buffer (50% Licor blocking buffer (Li-Cor, Lincoln, NE) with 50% TBS+0.1% Tween 20 (TBST)).

    Immunofluorescence:

    Article Title: The critical role of the proto-oncogene c- Kit in TSC renal cystogenesis
    Article Snippet: The following antibodies were utilized for immunofluorescence (IF) or immunohistochemistry (IHC) and Western blots (WB): (1) p-ribosomal S6--IHC (1:100) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (2) c-KIT antibody--IF (1:100) and WB (1:1000) (Cell Signaling Technology; Danvers, MA); (3) pERK1/2 IHC (1:300) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (4) pRSK1 IHC (1:100) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (5) ser1798 p-TSC2 IF (1:75) and WB (1:800) (ThermoFisher Scientific, Eugene, OR); and (6) pAKT IHC (1:50) and WB (1:1000) (Cell Signaling Technology, Danvers, MA). .. The following antibodies were used for immunofluorescence studies: (1) Monoclonal H + -ATPase (1:50; Santa Cruz Biotechnology); (2) H + -ATPase (1:75; our lab); (3) PCNA (1:50; Santa Cruz Biotechnology); and (4) AQP2 (1:50; Santa Cruz Biotechnology). .. M-1 cells derived from the renal cortex of a SV40 early region transgenic mouse were purchased from American Type Culture Collection (ATCC, Manassas, VA).

    Article Title: The critical role of the proto-oncogene c-Kit in TSC renal cystogenesis.
    Article Snippet: The following antibodies were utilized for immunofluorescence (IF) or immunohistochemistry (IHC) and Western blots (WB): (1) p-ribosomal S6--IHC (1:100) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (2) c-KIT antibody--IF (1:100) and WB 14 EMBO Molecular Medicine © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply (1:1000) (Cell Signaling Technology; Danvers, MA); (3) pERK1/2 IHC (1:300) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (4) pRSK1 IHC (1:100) and WB (1:1000) (Cell Signaling Technology, Danvers, MA); (5) ser1798p-TSC2 IF (1:75) and WB (1:800) (ThermoFisher Scientific, Eugene, OR); and (6) pAKT IHC (1:50) and WB (1:1000) (Cell Signaling Technology, Danvers, MA). .. The following antibodies were used for immunofluorescence studies: (1) Monoclonal H+-ATPase (1:50; Santa Cruz Biotechnology); (2) H+-ATPase (1:75; our lab); (3) PCNA (1:50; Santa Cruz Biotechnology); and (4) AQP2 (1:50; Santa Cruz Biotechnology). .. M-1 cells derived from the renal cortex of a SV40 early region transgenic mouse were purchased from American Type Culture Collection (ATCC, Manassas, VA).

    other:

    Article Title: A Novel Human Distal Tubuloid-on-a-Chip Model for Investigating Sodium and Water Transport Mechanisms
    Article Snippet: , AQP2 , Santa Cruz Biotechnology , Rabbit , — , Sc-515770 , 1:300.



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    Image Search Results


    Structure, function, and regulatory mechanisms of CRL5, along with the upstream and downstream signaling pathways of CUL5 in different tumors.

    Journal: Frontiers in Medicine

    Article Title: CUL5-mediated ubiquitination in cancer cell therapy: context-dependent roles, molecular networks, and emerging therapeutic avenues

    doi: 10.3389/fmed.2026.1775447

    Figure Lengend Snippet: Structure, function, and regulatory mechanisms of CRL5, along with the upstream and downstream signaling pathways of CUL5 in different tumors.

    Article Snippet: Similarly, CUL5 ubiquitinates AQP2, further influencing vascular dynamics ( , ), suggesting broader roles in fluid homeostasis during vascular development.

    Techniques: Protein-Protein interactions

    Regulation and downstream effects of CUL5 in CAR-T cells.

    Journal: Frontiers in Medicine

    Article Title: CUL5-mediated ubiquitination in cancer cell therapy: context-dependent roles, molecular networks, and emerging therapeutic avenues

    doi: 10.3389/fmed.2026.1775447

    Figure Lengend Snippet: Regulation and downstream effects of CUL5 in CAR-T cells.

    Article Snippet: Similarly, CUL5 ubiquitinates AQP2, further influencing vascular dynamics ( , ), suggesting broader roles in fluid homeostasis during vascular development.

    Techniques:

    Connexin 43 (Cx43) expression in different cellular populations of renal tissue sections from control (C) and diabetic patients (DM). Representative photomicrographs show Cx43 (Cx43, green) expression in the kidneys of control and diabetic groups co-localized with ( a ) aquaporin 2 (AQP2, red), a marker for collecting duct epithelium; ( b ) synaptopodin, a marker for podocytes (synaptopodin, red) and ( c ) PDGFRB, a marker for pericytes and mesangial cells (PDGFRB, red). g—glomerulus; arrowheads indicate positive Cx43 staining. Nuclei are stained blue. Scale bar = 50 µm (refers to all).

    Journal: International Journal of Molecular Sciences

    Article Title: Connexin 43 and Pannexin 1 in Renal Cell Populations in Diabetic Kidney Disease

    doi: 10.3390/ijms27052152

    Figure Lengend Snippet: Connexin 43 (Cx43) expression in different cellular populations of renal tissue sections from control (C) and diabetic patients (DM). Representative photomicrographs show Cx43 (Cx43, green) expression in the kidneys of control and diabetic groups co-localized with ( a ) aquaporin 2 (AQP2, red), a marker for collecting duct epithelium; ( b ) synaptopodin, a marker for podocytes (synaptopodin, red) and ( c ) PDGFRB, a marker for pericytes and mesangial cells (PDGFRB, red). g—glomerulus; arrowheads indicate positive Cx43 staining. Nuclei are stained blue. Scale bar = 50 µm (refers to all).

    Article Snippet: , Anti-Aquaporin 2/AQP2 (E-2) , sc-515770 , Mouse , 1:50 , Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA.

    Techniques: Expressing, Control, Marker, Staining

    Pannexin 1 (PANX1) expression in different cellular populations of renal tissue sections from control (C) and diabetic (DM) patients. Representative photomicrographs show PANX1 (PANX1, green) expression in the kidneys of control and diabetic groups co-localized with ( a ) aquaporin 2 (AQP2, red), a marker for collecting duct epithelium; ( b ) nephrin (nephrin, red) a marker for podocytes. Arrowheads indicate positive PANX1 staining. Nuclei are stained blue. Scale bar = 50 µm (refers to all). g—glomerulus.

    Journal: International Journal of Molecular Sciences

    Article Title: Connexin 43 and Pannexin 1 in Renal Cell Populations in Diabetic Kidney Disease

    doi: 10.3390/ijms27052152

    Figure Lengend Snippet: Pannexin 1 (PANX1) expression in different cellular populations of renal tissue sections from control (C) and diabetic (DM) patients. Representative photomicrographs show PANX1 (PANX1, green) expression in the kidneys of control and diabetic groups co-localized with ( a ) aquaporin 2 (AQP2, red), a marker for collecting duct epithelium; ( b ) nephrin (nephrin, red) a marker for podocytes. Arrowheads indicate positive PANX1 staining. Nuclei are stained blue. Scale bar = 50 µm (refers to all). g—glomerulus.

    Article Snippet: , Anti-Aquaporin 2/AQP2 (E-2) , sc-515770 , Mouse , 1:50 , Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA.

    Techniques: Expressing, Control, Marker, Staining